The majority of human genome can be transcribed as RNA molecules with less than 2% of them encoding proteins. The major types, long RNA molecules (>200 nt), controls numerous aspects of human biological processes. However, our current understanding remains scarce and is limited by the low processivity of reverse transcriptases, a group of enzymes that use RNA as the template to synthesize DNA (cDNA). Recently, our lab discovered an ultra-processive reverse transcriptase, dubbed MarathonRT, that will transform our ability to thoroughly and accurately interrogate transcriptomes. My research focuses on this remarkable enzyme, and I am using a multidisciplinary approach aiming at developing a robust tool for RNA biology.