• Research in macromolecular structure and function

    In our studies of RNA and protein enzymes, we use technical approaches that include pre-steady-state kinetics, analytical ultracentrifugation, crystallography, microscopy and cell culture.
  • Instrumentation in the Pyle Lab

    State-of-the-art instrumentation on site facilitates all of our scientific investigations.
  • Three views of RIG-I bound to duplex RNA

    The RIG-I innate immune receptor is a "surveillance protein" that helps us detect and combat viral infections. We solved its structure and showed how viral RNA (gold) is recognized by the RIG-I protein (green).
  • DNA-RNA synthesis and mechanistic analysis

    We make our own modified DNA and RNA, using chemical synthesis to produce unusual nucleic acids for the study of molecular recognition.
  • Large noncoding RNAs have elaborate structures

    Our studies on the group II self-splicing intron shows that large noncoding RNAs can adopt elaborate tertiary structures, enabling them to catalyze complex reactions.
  • Rapid quench instrument for analysis of enzyme kinetics

    To understand the molecular mechanisms of catalysis by our enzymes, and to probe their structures, we employ a diversity of enzymological techniques.



Welcome to the Pyle Lab.  We explore RNA Biology, studying the molecular interactions important for RNA structures and the activities of RNA-protein machines.  Using tools that range from crystallography to cell culture, we seek to understand the impact of RNA architecture and dynamics on the life of the cell. 

Spotlight Publications

Native Purification and Analysis of Long RNAs
Methods in Enzymology   2015 (558) : Epub ahead of print.