The mechanism of genome invasion
We determined the 2.8 Å cryo-EM structure of a group IIC intron with its MarathonRT maturase and DNA target to understand the molecular recognition mechanisms behind genome invasion by this mobile retroelement.
Research in macromolecular structure and function
In our studies of RNA and protein enzymes, we use technical approaches that include pre-steady-state kinetics, analytical ultracentrifugation, crystallography, microscopy and cell culture.
Instrumentation in the Pyle Lab
State-of-the-art instrumentation on site facilitates all of our scientific investigations.
Three views of RIG-I bound to duplex RNA
The RIG-I innate immune receptor is a "surveillance protein" that helps us detect and combat viral infections. We solved its structure and showed how viral RNA (gold) is recognized by the RIG-I protein (green).
DNA-RNA synthesis and mechanistic analysis
We make our own modified DNA and RNA, using chemical synthesis to produce unusual nucleic acids for the study of molecular recognition.
Large noncoding RNAs have elaborate structures
Our studies on the group II self-splicing intron shows that large noncoding RNAs can adopt elaborate tertiary structures, enabling them to catalyze complex reactions.
Rapid quench instrument for analysis of enzyme kinetics
To understand the molecular mechanisms of catalysis by our enzymes, and to probe their structures, we employ a diversity of enzymological techniques.